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In IIS 7.5 and greater, you have to assign permissions to the user which runs your application pool. Usually, when you create a web application in IIS, IIS also automatically creates a new application pool with the same name. As you can see in the sample below, I created a new application named "TestApp".
In IIS, the object model isn't as crazy as the tree in IIS Manager presents. Sites have Applications. Applications have virtual directories. That's that. Deeply nested relationships are modelled by storing the paths. Even though IIS Manager shows VDir1 as having an application inside it, the reality is that the application belongs to the site.
IIS_WPG automatically gets access to these when a new website is created. Copy/paste permits the process group to gain these permissions. Otherwise, you have to specify that the group should have read/execute permissions. Extra, completely gratuitous background. The bad days: worker processes roam the countryside
Give proper permissions on the Permissions for Authenticated Users box on the lower end if they're not checked already. Click OK twice to close the dialog box. It should take effect immediately, but if you want to be sure, you can restart IIS for your web application.
Using Windows 7 Home Premium with IIS 7.5 Express installed I cannot find the 'IIS Manage Users' function on the localhost / webserver level. I have been in Control Panel --> Programs and Features --> Turn Windows Features On/Off --> and pretty much enabled/installed everything for IIS and it's security sub folder but no such functionality can be found within IIS after restarting it.
Complete the following steps to re-configure IIS Version 9.x: Click the Tools> Internet Information Services (IIS) Manager within the Server Manager screen. This action starts the IIS application, and creates a new virtual directory for the website instance that you intend to use with WebSphere Application Server.
Setting IIS Process Identity for IIS 7.0 on Windows Server 2008 or Windows Vista. Click Start, then All Programs, and click Internet Information Services (IIS) 7 Manager. In Internet Information Services (IIS) Manager, expand <computer name>(User account) and click Application Pools. Right-click an application pool and click View Applications ...
Setting IIS Process Identity for IIS 7.0 on Windows Server 2008 or Windows Vista. Click Start, then All Programs, and click Internet Information Services (IIS) 7 Manager. In Internet Information Services (IIS) Manager, expand <computer name>(User account) and click Application Pools. Right-click an application pool and click View Applications ...

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